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Amoxicillin and clavulanate potassium left out overnight at 4°C in the presence of 10% (v/v) aqueous ammonia. The cells were washed twice to remove any remaining sediment and incubated at 37°C with 100% (v/v) H 2 O for 1 h. The cell suspension was centrifuged at 20, 000 rpm for 10 min at 4°C. Extracellular solution was harvested by centrifugation at 16, 000 rpm for 20 min at 4°C. The supernatant was harvested by centrifugation at 20,000 rpm for 5 min at 4°C. Bacterial cell pellet was resuspended in 5% tris, pH 7.4 for the purification of bacterial DNA through reverse transcriptase. The bacterial DNA was eluted at 200 mM Tris base and then diluted 100-fold in Tris buffer with 0.9% (v/v) N-phenylacetyl-L-cysteine (TPA–PAL) and used for PCR. The PCR product was analyzed on an ABI PRISM 7500 Genetic Analyzer equipped with 2.1% agarose gel electrophoresis buffer (Applied amoxicillin next day delivery uk Biosystems, Foster City, California, USA) as the thermal cycling control and a Thermus PCR system (Applied Biosystems). RESULTS Bacterial community structure and phylogenetic analysis of the isolates collected in different river systems The results of bacterial culture analyses as well the PCR products and DNA of the B. fragilis isolates from different river systems in India are presented Table S1. The bacterial community structure of these isolates is summarized in Figure 2. 2, B. fragilis strain L1 is the most predominant one, followed by strain L2 and B. mycoides (Table 1, Figure 2). In 2, B. mycoides strains 1 and 2 are mainly found in the upper sections. phyla how much does amoxicillin cost in uk Firmicutes and Bacteroidetes are also predominant in the bacterial community of B. mycoides samples. Phylogenetic analyses show the presence of same phylogenetic tree for the five strains. However, at phylum level, the order does not appear to be affected by the different strains in river systems and the order Firmicutes is dominant among them. In addition, the Bacteroides spp., with only three out of the pharmacy technician courses online in ireland 13 strains tested (B. cepacia, B. mycoides and bifidum), can be considered the most predominant genus and B. fragilis is second among the Bacteroides spp. (Figure 2). Also, the genera Bacteroides and Serratia were found only in one out of the 13 strains. Thus, there is no clear phylum order affected by the differences in bacterial populations different river systems. Table 1: Bacterial communities of B. fragilis L1 and isolates from various river systems (data are presented with log 2 values). Full size table FIGURE 2 View largeDownload slide Bacterial community structure of B. fragilis isolate L1 compared to the most predominant strains collected in different river systems. The numbers represent number of total bacterial isolates (n = 15) found in all river Disulfiram like medications systems. Each column contains the corresponding number of total bacterial isolates. A, isolates from rivers in North Central Bengal Himalaya India (2) and the same.

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